PhD Student (d/f/m) Translational Cardiovascular Research

Working at the Charité

The laboratory studying metabolic thrombosis risk headed by Dr. Marco Witkowski is looking for a part-time PhD student (65%) for at least three years (options for pro-longation are in
place) with a background in biology or medicine and relevant previous scientific experience.Details on work hours per week can be discussed individually according to the candidate’s
need. Our research combines clinical observational and interventional studies, metabolomics and vascular biology/thrombosis research (both, in vitro and in vivo models). We are a team
of collaborative and innovative researchers (clinicians and basic scientists) that share the passion for research inclduing clinical studies and basic understanding of disease
processes.

Job overview

• Design of in vitro experiments to assess effects of specific metabolites on thrombosis potential (e.g. platelet function and coagulation factors)
• Mass spectrometry assays to measure specific metabolites in clinical samples/mouse samples in collaboration with a working group at the Max-Planck-Institute Golm (training will be
  provided)
• Cell culture experiments using vascular cells, including endothelial cells
• Use of clinical samples to perform ex vivo assays (whole blood, platelets, PBMCs)
• In vivo experiments using mice, including DNA/RNA analysis, protein assays, immunofluorescence
• Statistical analysis of data, presentation of results at international conferences, manuscript writing

We are looking for

• Ihr wissenschaftliches Hochschulstudium, z. B. Biologie, Biotechnologie, Molekularmedizin/-biologie, Biochemie, etc. haben Sie mit einem Master abgeschlossen und qualifizieren sich
  für eine PhD-Arbeit
• Vielleicht haben Sie bereits in der kardiovaskulären Forschungb gearbeitet, idealerweise in der Gefäßbiologie bzw. Thromboseforschung,
• Sie beherrschen die Standardtechniken der Molekularbiologie (enzyme assays, DNA/RNA isolation, rtPCR, Zellkultur, Proteinassays (z.B. western blot)
• Sie haben Erfahrung in Immunfluoreszenz und Histologie mit Datenanalyse, in der Durchflusszytometrie, Design von Flow-panels und mit Mausmodellen (idealerweise FELASA-B-Zertifikat
  oder äquivalent)
• Außerdem haben Sie bestenfalls mit einem Massenspektrometer gearbeitet
• Sie arbeiten unabhängig, routiniert und gut organisiert, übernehmen Verantwortung und leben Teamplay
• Sie möchten Ihren Horizont kontinuierlich weiterentwickeln und fühlst Dich wohl in einem motivierten Forschungsumfeld
• Sie sprechen fließend Deutsch oder Englisch, idealerweise bringen Sie ebenfalls Coding-Fähigkeiten (z.B. R) mit

Advantages at the Charité

• A relaxed but highly professional collaboration in a familiar, motivated, interdisciplinary team
• A future-oriented, varied and challenging job with a high degree of personal responsibility and personal freedom of action
• Multicultural team events, well-structured onboarding with nice colleagues
• Extensive free continuing education and training opportunities up to and including university studies
• Discounts on many employee offers for shopping, travel, sports
• Our company pension scheme provides our employees with additional security in old age
• Charité – Universitätsmedizin Berlin is certified for a family-friendly balance between private life, family and work or studies – you can find further information here

Job Information

• Pay scale E13 according to TVöD VKA-K . The grouping takes place under consideration of the qualification, the respective experience level is calculated from the
  completed professional years. The annual salary (gross) is given for a full-time position without annual bonus, allowances and additional services. You can find the collective
  agreement here
• The working time is part-time with 25.36 hours per week
• The application deadline is on: 29.09.2024
• Reference number: 3372